Highest trk B mRNA expression in the entorhinal cortex among hippocampal subregions in the adult rat: contrasting pattern with BDNF mRNA expression
Identifieur interne : 003A68 ( Main/Exploration ); précédent : 003A67; suivant : 003A69Highest trk B mRNA expression in the entorhinal cortex among hippocampal subregions in the adult rat: contrasting pattern with BDNF mRNA expression
Auteurs : Wataru Tokuyama [Japon] ; Takanori Hashimoto [Japon] ; Yue Xin Li [Japon] ; Hiroyuki Okuno [Japon] ; Yasushi Miyashita [Japon]Source :
- Molecular Brain Research [ 0169-328X ] ; 1998.
English descriptors
- Teeft :
- Assay, Bdnf, Bdnf mrna, Bdnf mrna expression level, Bdnf protein, Bdnfrtrkb, Coamplification, Coamplification analysis, Coamplification method, Cyclophilin, Cyclophilin mrna, Dentate gyrus, Differential expression patterns, Endogenous, Entorhinal, Entorhinal cortex, Error bars, Expression levels, Expression patterns, Granule cells, Hilar region, Hippocampal, Hippocampal formation, Hippocampal subregions, Hippocampus, Hybridization, Image analyzer, Isoforms, Molecular brain research, Mrna, Mrna content, Mrna contents, Mrna expression, Mrna expression level, Mrna expression levels, Mrna levels, Neuron, Neurosci, Neurotrophic, Neurotrophic factor, Present study, Primer, Principal neurons, Quantification, Receptor, Regional differences, Relative mrna levels, Ribonuclease, Ribonuclease protection assay, Ribonuclease protection assays, Statistical analysis, Stratum radiatum, Subregions, Target genes, Tissue dissection, Tokuyama, Total rnas, Trkb, Trkb receptors.
Abstract
Abstract: Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, regulate synaptic functions in the hippocampus of the adult rodent. In previous studies, in situ hybridization methods have been used to evaluate regional differences in BDNF and trkB mRNA expression levels in hippocampal subregions. However, these studies have failed to reach consensus regarding the regional differences in the mRNA expression levels. In the present study, we quantitated mRNA expression levels using two different methods, ribonuclease protection assays and a quantitative reverse-transcription polymerase chain reaction technique, in four hippocampal subregions: the entorhinal cortex, dentate gyrus (DG), CA3 and CA1. These two methods yielded the same results. We found that BDNF and trkB mRNA expression levels did not covary in the four subregions. BDNF and full length trkB (trkB FL) mRNA in the entorhinal cortex and the DG show contrasting expression patterns. The expression level of BDNF mRNA was highest in the DG among the hippocampal subregions and low in the entorhinal cortex and the CA1, whereas the trkB FL mRNA expression level was highest in the entorhinal cortex, low in the DG and lowest in the CA3. These results suggest regional differences in BDNF/TrkB signaling for maintenance and modifiability of neuronal connections in the hippocampal formation.
Url:
DOI: 10.1016/S0169-328X(98)00261-7
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001F80
- to stream Istex, to step Curation: 001F80
- to stream Istex, to step Checkpoint: 001277
- to stream Main, to step Merge: 003B17
- to stream Main, to step Curation: 003A68
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Highest trk B mRNA expression in the entorhinal cortex among hippocampal subregions in the adult rat: contrasting pattern with BDNF mRNA expression</title><author><name sortKey="Tokuyama, Wataru" sort="Tokuyama, Wataru" uniqKey="Tokuyama W" first="Wataru" last="Tokuyama">Wataru Tokuyama</name></author><author><name sortKey="Hashimoto, Takanori" sort="Hashimoto, Takanori" uniqKey="Hashimoto T" first="Takanori" last="Hashimoto">Takanori Hashimoto</name></author><author><name sortKey="Li, Yue Xin" sort="Li, Yue Xin" uniqKey="Li Y" first="Yue Xin" last="Li">Yue Xin Li</name></author><author><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name></author><author><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:2C14BD207C88068E5005E776ACB41076AC51A492</idno><date when="1998" year="1998">1998</date><idno type="doi">10.1016/S0169-328X(98)00261-7</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-QS4F0J80-R/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001F80</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001F80</idno><idno type="wicri:Area/Istex/Curation">001F80</idno><idno type="wicri:Area/Istex/Checkpoint">001277</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001277</idno><idno type="wicri:doubleKey">0169-328X:1998:Tokuyama W:highest:trk:b</idno><idno type="wicri:Area/Main/Merge">003B17</idno><idno type="wicri:Area/Main/Curation">003A68</idno><idno type="wicri:Area/Main/Exploration">003A68</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Highest trk B mRNA expression in the entorhinal cortex among hippocampal subregions in the adult rat: contrasting pattern with BDNF mRNA expression</title><author><name sortKey="Tokuyama, Wataru" sort="Tokuyama, Wataru" uniqKey="Tokuyama W" first="Wataru" last="Tokuyama">Wataru Tokuyama</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Physiology, University of Tokyo School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Hashimoto, Takanori" sort="Hashimoto, Takanori" uniqKey="Hashimoto T" first="Takanori" last="Hashimoto">Takanori Hashimoto</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Mind Articulation Project, ICORP, JST, Yushima, Tokyo 113-0034</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Li, Yue Xin" sort="Li, Yue Xin" uniqKey="Li Y" first="Yue Xin" last="Li">Yue Xin Li</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Mind Articulation Project, ICORP, JST, Yushima, Tokyo 113-0034</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Physiology, University of Tokyo School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name><affiliation wicri:level="1"><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Physiology, University of Tokyo School of Medicine, Hongo 7-3-1, Bunkyo-ku, Tokyo 113-0033</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Molecular Brain Research</title><title level="j" type="abbrev">BRESM</title><idno type="ISSN">0169-328X</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1998">1998</date><biblScope unit="volume">62</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="206">206</biblScope><biblScope unit="page" to="215">215</biblScope></imprint><idno type="ISSN">0169-328X</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0169-328X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Assay</term><term>Bdnf</term><term>Bdnf mrna</term><term>Bdnf mrna expression level</term><term>Bdnf protein</term><term>Bdnfrtrkb</term><term>Coamplification</term><term>Coamplification analysis</term><term>Coamplification method</term><term>Cyclophilin</term><term>Cyclophilin mrna</term><term>Dentate gyrus</term><term>Differential expression patterns</term><term>Endogenous</term><term>Entorhinal</term><term>Entorhinal cortex</term><term>Error bars</term><term>Expression levels</term><term>Expression patterns</term><term>Granule cells</term><term>Hilar region</term><term>Hippocampal</term><term>Hippocampal formation</term><term>Hippocampal subregions</term><term>Hippocampus</term><term>Hybridization</term><term>Image analyzer</term><term>Isoforms</term><term>Molecular brain research</term><term>Mrna</term><term>Mrna content</term><term>Mrna contents</term><term>Mrna expression</term><term>Mrna expression level</term><term>Mrna expression levels</term><term>Mrna levels</term><term>Neuron</term><term>Neurosci</term><term>Neurotrophic</term><term>Neurotrophic factor</term><term>Present study</term><term>Primer</term><term>Principal neurons</term><term>Quantification</term><term>Receptor</term><term>Regional differences</term><term>Relative mrna levels</term><term>Ribonuclease</term><term>Ribonuclease protection assay</term><term>Ribonuclease protection assays</term><term>Statistical analysis</term><term>Stratum radiatum</term><term>Subregions</term><term>Target genes</term><term>Tissue dissection</term><term>Tokuyama</term><term>Total rnas</term><term>Trkb</term><term>Trkb receptors</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Brain-derived neurotrophic factor (BDNF) and its receptor, TrkB, regulate synaptic functions in the hippocampus of the adult rodent. In previous studies, in situ hybridization methods have been used to evaluate regional differences in BDNF and trkB mRNA expression levels in hippocampal subregions. However, these studies have failed to reach consensus regarding the regional differences in the mRNA expression levels. In the present study, we quantitated mRNA expression levels using two different methods, ribonuclease protection assays and a quantitative reverse-transcription polymerase chain reaction technique, in four hippocampal subregions: the entorhinal cortex, dentate gyrus (DG), CA3 and CA1. These two methods yielded the same results. We found that BDNF and trkB mRNA expression levels did not covary in the four subregions. BDNF and full length trkB (trkB FL) mRNA in the entorhinal cortex and the DG show contrasting expression patterns. The expression level of BDNF mRNA was highest in the DG among the hippocampal subregions and low in the entorhinal cortex and the CA1, whereas the trkB FL mRNA expression level was highest in the entorhinal cortex, low in the DG and lowest in the CA3. These results suggest regional differences in BDNF/TrkB signaling for maintenance and modifiability of neuronal connections in the hippocampal formation.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><country name="Japon"><region name="Région de Kantō"><name sortKey="Tokuyama, Wataru" sort="Tokuyama, Wataru" uniqKey="Tokuyama W" first="Wataru" last="Tokuyama">Wataru Tokuyama</name></region><name sortKey="Hashimoto, Takanori" sort="Hashimoto, Takanori" uniqKey="Hashimoto T" first="Takanori" last="Hashimoto">Takanori Hashimoto</name><name sortKey="Li, Yue Xin" sort="Li, Yue Xin" uniqKey="Li Y" first="Yue Xin" last="Li">Yue Xin Li</name><name sortKey="Miyashita, Yasushi" sort="Miyashita, Yasushi" uniqKey="Miyashita Y" first="Yasushi" last="Miyashita">Yasushi Miyashita</name><name sortKey="Okuno, Hiroyuki" sort="Okuno, Hiroyuki" uniqKey="Okuno H" first="Hiroyuki" last="Okuno">Hiroyuki Okuno</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003A68 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 003A68 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= MersV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:2C14BD207C88068E5005E776ACB41076AC51A492
|texte= Highest trk B mRNA expression in the entorhinal cortex among hippocampal subregions in the adult rat: contrasting pattern with BDNF mRNA expression
}}
| This area was generated with Dilib version V0.6.33. |  |